REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Mouse CD45 (clone 30-F11)	Biolegend	Cat# 103126; RRID:AB_493535
Anti-Mouse CCR3 (clone J073E5)	Biolegend	Cat# 144510; RRID:AB_2561609
Anti-Mouse SiglecE (clone M1304A01)	Biolegend	Cat# 677106; RRID:AB_2566171
Anti-Mouse CD45.2 (clone 104)	Biolegend	Cat# 109814; RRID:AB_389211
Anti-Mouse CD45.2 (clone 104)	Biolegend	Cat# 109831; RRID:AB_10900256
Anti-Mouse CXCR2 (clone SA044G4)	Biolegend	Cat# 149304; RRID:AB_2565692
Anti-Mouse CD11b (clone M1/70)	BD Biosciences	Cat# 557657; RRID:AB_396772
Anti-Mouse Ly-6G (clone1A8)	BD Biosciences	Cat# 551461; RRID:AB_394208
Anti-Mouse Ly-6G (clone1A8)	BD Biosciences	Cat# 560599; RRID:AB_1727560
Anti-Mouse Ly-6G (clone1A8)	Biolegend	Cat# 127618; RRID:AB_1877261
Anti-Mouse Ly-6G (clone1A8)	Biolegend	Cat# 127602; RRID:AB_1089180
Anti-Mouse Ly-6G (clone1A8)	Biolegend	Cat# 127643; RRID:AB_2565971
Anti-Mouse SiglecF (clone E50-2440)	BD Biosciences	Cat# 564514; RRID:AB_2738833
Anti-Mouse SiglecF (clone E50-2440)	BD Biosciences	Cat# 552125; RRID:AB_394340
Anti-Mouse SiglecF (clone E50-2440)	BD Biosciences	Cat# 562681; RRID:AB_2722581
Anti-Mouse SiglecF (clone E50-2440)	BD Biosciences	Cat# 565526; RRID:AB_2739281
Anti-Mouse B220 (clone RA3-6B2)	BD Biosciences	Cat# 552772; RRID:AB_394458
Anti-Mouse CD90.2 (clone 53-2.1)	BD Biosciences	Cat# 561642; RRID:AB_10895975
Anti-Mouse CD45.1 (clone A20)	BD Biosciences	Cat# 560578; RRID:AB_1727488
Anti-Mouse CD16/32 (clone 93), TruStain FcX	Biolegend	Cat# 101320; RRID:AB_1574975
Biological Samples
Lungs from KP1.9 tumor-bearing or tumor-free mice	Mikael Pittet lab	N/A
Blood from KP1.9 tumor-bearing mice	Mikael Pittet lab	N/A
Spleens from KP1.9 tumor-bearing mice	Mikael Pittet lab	N/A
Bone marrow from KP1.9 tumor-bearing mice	Mikael Pittet lab	N/A
Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice	Mikael Pittet lab	N/A
Chemicals, Peptides, and Recombinant Proteins
7-aminoactinomycin D	Sigma	Cat# A9400
Collagenase type I	Worthington	Cat# LS004197
ACK lysis buffer	Lonza	Cat# 10-548E
Flow Cytometry Absolute Count Standard	Bangs Laboratories	Cat# 580
Histopaque-1077	Sigma-Aldrich	Cat# 10771
Histopaque-1119	Sigma-Aldrich	Cat# 11191
Critical Commercial Assays
Fixation/Permeabilization Solution Kit	BD Biosciences	Cat# 554714
Zombie Aqua Fixable Viability Kit	Biolegend	Cat# 423102
ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit	Vector Laboratories	Cat# MP-5444
BLOXALL endogenous peroxidase blocking solution	Vector Laboratories	Cat# SP6000
DAB Quanto Chromogen and Substrate	ThermoFisher Scientific	Cat# TA-060-QHDX
Deposited Data
Neutrophil single cell RNA sequencing data	(Engblom et al., 2017; Zilionis et al., 2019)	GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019)
Experimental Models: Cell Lines
Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse	Alfred Zippelius	N/A
Experimental Models: Organisms/Strains
Mouse: C57BL/6J mice	Jackson Laboratory	Cat# 000664; RRID:IMSR_JAX:000664
Mouse: B6.SJL-PtprcaPepcb/BoyJ	Jackson Laboratory	Cat# 002014; RRID:IMSR_JAX:002014
Software and Algorithms
FlowJo v.8.8.7 and 10.4	FlowJo, LLC	RRID:SCR_008520
BD FACSDiva	BD Biosciences	RRID:SCR_001456
GraphPad Prism v.8	GraphPad Prism	RRID:SCR_002798
Python 3.6.7	Anaconda	https://www.anaconda.com/products/individual
NanoZoomer Digital Pathology (NDP.view2)	Hamamatsu	https://www.hamamatsu.com/eu/en/product/type/U12388-01/index.html
Aperio ImageScope	Leica	https://www.leicabiosystems.com/digital-pathology/manage/aperio-imagescope/
Scanpy v. 1.4.4.post1	(Wolf et al., 2018)	https://scanpy.readthedocs.io/en/1.4.4.post1/