Figure 2.

TSG can prevent HUVEC aging. (A) Representative images of senescence-associated β-gal staining in HUVEC pretreated with TSG under H₂O₂ induction. (B) Quantification of SA-β-gal positive cell number from three biological replicates. For each replicate, at least 5 random microscopic fields were counted. (C–E) Representative images of WB analysis and the semi-quantification of PAI-1 and p21 in HUVEC pretreated with TSG under H₂O₂ induction. The values are expressed as the mean ± SD (n = 3). *p <0.05 and **p < 0.01 vs. control, ^# p < 0.05 and ^## p < 0.01 vs. H₂O₂.