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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Mol Cancer Res. 2020 Mar 17;18(6):891–902. doi: 10.1158/1541-7786.MCR-19-1208

Figure 7.

Figure 7.

Genetic analysis of the selective cleavage activity of the R34G-targeting RNP in a wildtype versus mutated NRF2 sequence. This experiment was conducted using three different cell lines: (A) A549 parental cells, (B) A549 R34G-6 cells, (C) H1703 parental cells. Each cell line was transfected using three different conditions: 1) equimolar concentration of duplexed guide RNA to Cas9 (top panel); 2) five times the amount of duplexed guide RNA to Cas9 (middle panel); 3) five times the amount of single guide RNA to Cas9 (bottom panel). Gene editing activity was assessed by bulk sequence analysis using TIDE. Each panel represents the data output of indel formation from TIDE.