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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Traffic. 2020 Jun 16;21(8):534–544. doi: 10.1111/tra.12737

FIGURE 1.

FIGURE 1

FP cytotoxicity in bacteria can be reduced by modifying the protein termini. E. coli cells of strain DH10B were transformed with the indicated FP expression constructs, and colony areas were measured. Average colony areas are plotted in arbitrary units (a.u.). Bars indicate SEM. Statistical significance was measured using Welch’s t-test. Four asterisks indicate significance at P < 0.0001, and “ns” indicates no significant difference. The table at the bottom lists the N- and C-terminal peptides of the FPs, with the dashes indicating amino acids that are missing from one of the two FPs in each pair.