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. 2020 Sep 4;11:1074. doi: 10.3389/fphys.2020.01074

Figure 2.

Figure 2

The expression of growth arrest and DNA damage-inducible beta (GADD45B) was increased in the kidneys of db/db mice and HG-induced HK-2 cells. (A) Heat map of the 1,194 differentially expressed genes (the logarithm of the fold change to base 2 > 2) and the right part is the enlarged view containing GADD45B. (B) The logarithm of the fold change to base 2 of GADD45B expression was determined by RNA sequencing analysis. (C) Real-time PCR (RT-PCR) analysis of GADD45B messenger RNA (mRNA) expression in kidney samples, N = 9. (D) Representative images of immunohistochemical staining of GADD45B in kidney sections. Original magnification = 400. Scale bar = 100 μm. (E,F) Western blot bands and quantitative analysis of GADD45B protein expression in kidney samples, N = 6. (G,H) RT-PCR analysis of GADD45B mRNA expression in HK-2 cells, N = 3. (I–L) Western blot bands and quantitative analysis of GADD45B protein expression in HK-2 cells, N = 3. The data are presented as the mean ± SD. * p < 0.05.