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. 2020 Sep 4;11:1074. doi: 10.3389/fphys.2020.01074

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Copyright © 2020 Xue, Sun, Xu, Wang, Guo, Li, Cheng, Xu, Tang, Sun and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of GADD45B knockdown on renal tubular epithelial-mesenchymal transition (EMT) and apoptosis in HK-2 cells under high glucose (HG) conditions. (A) Representative images of immunofluorescence staining of GADD45B in HK-2 cells. Original magnification = 200. Scale bar = 200 μm. (B) Western blot bands showing E-cadherin, Vimentin, and α-smooth muscle actin (α-SMA) protein expression in HK-2 cells. (C) Quantitative analysis of (B), N = 3. (D) Western blot bands showing Bax, Bcl2, and cleaved Caspase 3 protein expression in HK-2 cells. (E) Quantitative analysis of (D), N = 3. The data are presented as the mean ± SD. ^* p < 0.05.