KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-GFP	Clontech	Cat#632381, RRID:AB_2313808
Rabbit anti-hexokinase antibody	Rockland	Cat#100–4159, RRID:AB_21991
Anti-mouse 800 secondary	LI-COR	Cat#926–32210, RRID:AB_621842
Anti-rabbit 680 secondary	LI-COR	Cat#926–68071, RRID:AB_10956166
Rat anti-tubulin	Serotec	Cat#MCA78G, RRID:AB_325005
Anti-rat 680 secondary	LI-COR	Cat#926–68076, RRID:AB_10956590
Chemicals, Peptides, and Recombinant Proteins
Lactimidomycin (LTM)	Millipore	Cat#506291
RNaseI	Ambion	Cat#AM2294
Sequencing grade modified trypsin	Promega	Cat#V5111
PMSF	Sigma	Cat#78830
cOmplete mini EDTA-free protease inhibitor cocktail	Roche	Cat#29384100
Acid-washed glass beads	Sigma	Cat#G8772
PBS Odyssey Blocking Buffer	LI-COR	Cat#927–40100
Superscript III	Thermo	Cat#18080044
SYBR green mix	Thermo	Cat#4309155
Critical Commercial Assays
Q5 Site Directed Mutagenesis kit	NEB	Cat#E0554S
TURBO DNA-free kit	Ambion	Cat#AM1907
Deposited Data
Raw and analyzed sequencing data	This study	GEO: GSE150375
Experimental Models: Organisms/Strains
All yeast strains are described in Table S5.	Brar-Ünal Lab and this study	N/A
Oligonucleotides
oGAB-2736 GFP-qPCR_f ctccggtgaaggtgaaggtg	IDT	N/A
oGAB-2737 GFP-qPCR_r aggttggccatggaactgg	IDT	N/A
oGAB-3301 PFY1-qPCR_f acggtagacatgatgctgagg	IDT	N/A
oGAB-3302 PFY1-qPCR_r acggttggtggataatgagc	IDT	N/A
oGAB-7864 HYP2-qPCR_f TGTCAAGGCTCCAGAAGGTGA	IDT	N/A
oGAB-7865 HYP2-qPCR_r CCCATAGCGGAGATGATGGT	IDT	N/A
Recombinant DNA
pÜB1/pFA6A-KanMX	Addgene	Cat#39296
pÜB189/pFA6A-KanMX-pCUP1 (derived from pFA6A)	Brar-Ünal Lab	N/A
pÜB731 (derived from pNH604)	Brar-Ünal Lab	N/A
Software and Algorithms
Bowtie2	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
MochiView	Homann and Johnson, 2010	http://www.johnsonlab.ucsf.edu/mochi/
ImageStudio Lite Software	LI-COR	https://www.licor.com/bio/products/software/image_studio_lite/index.html