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. 2020 Jul 30;127(8):997–1022. doi: 10.1161/CIRCRESAHA.120.317295

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© 2020 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 5. — Representative peptographs of extracts of ruptured vs stable human plaque segments.

A and B, Extracts of ruptured (red) and adjacent stable (blue) segments of 5 human carotid plaques were analyzed using the PROTOMAP protocol. The extracts were subjected to SDS-PAGE, and the gels were cut into 22 slices, each corresponding to a molecular weight range. After in-gel trypsin digestion, peptides were extracted, identified by tandem mass spectrometry, and spectral counts were aggregated over all 22 slices. A, Proteins with differential abundance of lower-molecular weight peptides in extracts of ruptured vs. stable segments: ceruloplasmin (CP), angiotensinogen (AGT), ITIH4 (inter-alpha-trypsin inhibitor heavy chain), GPLD1 (phosphatidylinositol-glycan-specific phospholipase D), FA5 (coagulation factor V), SERPIND1 (serpin family D member 1), LAMB2 (laminin subunit beta-2), and SVIL (supervillin). B, ECM (Extracellular matrix) proteins that are significantly less abundant in extracts of ruptured vs stable human plaque segments: ACAN (aggrecan core protein), FBLN5 (fibulin 5), FMOD (fibromodulin), HAPLN (hyaluronan and proteoglycan link protein 1), LAMA5 (laminin subunit alpha 5), MFAP4 (microfibril-associated glycoprotein 4), LTBP1 (latent-transforming growth factor beta-binding protein 1), and VCAN (versican). A and B, Horizontal bars in each peptograph portray the total spectral counts for protein-specific peptides in each of the 22 gel slices (mean±SEM; n=5). Gel-slice number is on the leftward y-axis; molecular weight of the gel slices (in kilodaltons) is on the rightward y-axis.