Table 2.
mRNA expression levels of Gli1, Gli2, and Axin2 in the bulb and bulge regions of the hair follicles were analyzed by real time-PCR. The relative mRNA expression of different genes is presented as fold changes
| Bulge region | Bulb region | |||||
|---|---|---|---|---|---|---|
| Gene | azelaic (25 mM) | minoxidil (100 µM) | azelaic (25 mM)/minoxidil (100 µM) | azelaic (25 mM) | minoxidil (100 µM) | azelaic (25 mm)/ minoxidil (100 µM) |
| Gli1 | 168.19±35.99* | 0.08±0.03 | 0.66±0.10 | 0.42±0.08 | 5.19±1.31* | 0.056±0.012 |
| Gli2 | 9.72±1.58* | 1.13±0.59 | 0.159±0.05 | 0.0034±0.0016 | 0.117±0.045 | 0.041±0.027 |
| Axin2 | 0.86±0.23 | 0.19±0.09 | 0.157±0.11 | 0.023±0.0077 | 5.37±0.32* | 0.021±0.005 |
Gli1 and Gli2 mRNA levels are statistically significant in azelaic treated bulge cells. Gli1 and Axin2 upregulated in minoxidil treated bulb cells (*P< 0.05 compared to control).