Fig 4. Determining the effect of single Mo amino acid mutations on the propagation of Mo protein-only recPrP^Sc.

(A) Table summarizing the effect of single Mo amino acid substitutions on the ability of BV/Mo PrP^C chimeras to propagate Mo protein-only recPrP^Sc. All constructs are based on the BV PrP^C backbone. (B) Western blots of experiments summarized in (A), showing three-round reconstituted sPMCA reactions using partially purified PrP^C substrates supplemented with PrP^0/0 BH and seeded with various seeds, as indicated. -PK = samples not subjected to proteinase K digestion; all other samples were proteolyzed.