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Western blots of sPMCA reactions using brain homogenates from glycosylation mutants G1, G2, or G3 as substrates, as indicated. Reactions were seeded with Mo protein-only recPrPSc or RML, as indicated. -PK = samples not subjected to proteinase K digestion; all other samples were proteolyzed. Mouse amino acid numbering scheme is used to show locations of N-linked glycans on Mo PrP.
