Figure 4.

The effect of A. baumannii instillation on the pulmonary histopathology, pulmonary permeability, and mortality among mice with different immune conditions. (A) Hematoxylin and eosin staining and Smith score analysis to evaluate the histopathological changes in lung tissues from mice in each experimental group were performed at 6 and 24 h after A. baumannii administration (original magnification ×200). (B) Lung edema was measured by the ratio of lung wet weight to dry weight (W/D) at 6 and 24 h of the induction of A. baumannii-associated pneumonia in mice with different immune conditions. n = 5 for each group at each time point. (C) The mortalities of mice in different experimental groups were analyzed seven days after the administration with A. baumannii (n = 5 for each group). Comparing the NIA and CIA group, the hazard ratio is 0.37 (95% confidence interval, 0.05–0.85; P = 0.066). CIA: Immune compromised infection; CIC: Immune compromised control; NIA: Normal immunity infection; NIC: Normal immunity control.