Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Caenorhabditis elegans) | daf-2 | WormBase | Y55D5A.5 | |
| Gene (Caenorhabditis elegans) | daf-16 | WormBase | R13H8.1 | |
| Gene (Caenorhabditis elegans) | pdr-1 | WormBase | K08E3.7 | |
| Gene (Caenorhabditis elegans) | ced-3 | WormBase | C48D1.2 | |
| Gene (Caenorhabditis elegans) | drp-1 | WormBase | T12E12.4 | |
| Genetic reagent (Caenorhabditis elegans) | him-8(e1489); ∆mtDNA(uaDf5)/+ | Caenorhabditis Genetics Center | RRID:WB-STRAIN:WBStrain00024106 | LB138 |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370) | Caenorhabditis Genetics Center | RRID:WB-STRAIN:WBStrain00004309 | CB1370 |
| Genetic reagent (Caenorhabditis elegans) | daf-16(mu86) | Caenorhabditis Genetics Center | RRID:WB-STRAIN:WBStrain00004840 | CF1038 |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); daf-16(mu86); muEx268 [ges-1p::GFP:daf-16(cDNA) + odr-1::RFP] | Caenorhabditis Genetics Center | RRID:WB-STRAIN:WBStrain00004876 | CF1827 |
| Genetic reagent (Caenorhabditis elegans) | pdr-1(gk448) | International C. elegans Gene Knockout Consortium | RRID:WB-STRAIN:WBStrain00036256 | VC1024 |
| Genetic reagent (Caenorhabditis elegans) | ced-3(ok2734) | International C. elegans Gene Knockout Consortium | RRID:WB-STRAIN:WBStrain00032755 | RB2071 |
| Genetic reagent (Caenorhabditis elegans) | drp-1(tm1108) | Shohei Mitani | RRID:WB-STRAIN:WBStrain00005196 | CU6372 |
| Genetic reagent (Caenorhabditis elegans) | bcIs39 V [lim-7p::ced-1::GFP + lin-15(+)] | Barbara Conradt | RRID:WB-STRAIN:WBStrain00026469 | MD701 |
| Genetic reagent (Caenorhabditis elegans) | tomm-20::mCherry | Sasha de Henau | TBDL58 | |
| Genetic reagent (Caenorhabditis elegans) | ∆mtDNA(uaDf5)/+ in Bristol nuclear background | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); ∆mtDNA(uaDf5)/+ | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); daf-16(mu86); ∆mtDNA(uaDf5)/+ | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-16(mu86); ∆mtDNA(uaDf5)/+ | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); pdr-1(gk448) | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); daf-16(mu86); pdr-1(gk448) | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); ced-3(ok2734) | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); daf-16(mu86); ced-3(ok2734) | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); drp-1(tm1108) | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); daf-16(mu86); drp-1(tm1108) | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); tomm-20::mCherry | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); daf-16(mu86); tomm-20::mCherry | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-16(mu86); tomm-20::mCherry | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Caenorhabditis elegans) | daf-2(e1370); bcIs39 V [lim-7p::ced-1::GFP + lin-15(+)] | This study | See Materials and methods: Genetic crosses and genotyping | |
| Genetic reagent (Escherichia coli) | HT115 strain expressing Y55D5A_391.b (daf-2) ORF plasmid clone | Ahringer Group, Source BioScience | 3318_Cel_RNAi_complete | See Materials and methods: Knockdown of gene expression |
| Sequence-based reagent | PCR primers used in this study | This study | See Materials and methods: Genetic crosses and genotyping; Quantification of mtDNA copy number and ∆mtDNA frequency | |
| Peptide, recombinant protein | BlpI restriction endonuclease | New England Biolabs | Cat#R0585L | See Materials and methods: Genetic crosses and genotyping |
| Chemical compound, drug | Isopropyl-β-D-thiogalactopyranoside | Research Products International | Cat#I56000-1 | See Materials and methods: Genetic crosses and genotyping |
| Chemical compound, drug | 4’,6-diamidino-2-phenylindole (DAPI) | Thermo Fisher Scientific | Cat#D1306 | |
| Chemical compound, drug | Paraformaldehyde | Electron Microscopy Sciences | Cat#15710 | |
| Chemical compound, drug | Levamisole | Fisher Scientific | Cat#AC187870100 | |
| Commercial assay, kit | DreamTaq Green DNA Polymerase | Thermo Fisher Scientific | Cat#EP0713 | |
| Commercial assay, kit | Seahorse XFe96 FluxPak | Agilent | Cat#102601–100 | |
| Other | Eppendorf 96-well twin.tec PCR plates | Fisher Scientific | Cat#951020303 | |
| Other | QX200 ddPCR EvaGreen Supermix | Bio-Rad | Cat#1864034 | |
| Other | Automated Droplet Generation Oil for EvaGreen | Bio-Rad | Cat#1864112 | |
| Other | DG32 Automated Droplet Generator Cartridges | Bio-Rad | Cat#1864108 | |
| Other | Droplet Reader Oil for ddPCR | Bio-Rad | Cat#1863004 | |
| Software, algorithm | QuantaSoft | Bio-Rad | Cat#1864011 | |
| Software, algorithm | Zen | Carl Zeiss Microscopy GmbH | RRID:SCR_013672 | |
| Software, algorithm | Prism eight for macOS | GraphPad Software, Inc | RRID:SCR_002798 | Version 8.1.2 |
| Software, algorithm | ImageJ | Wayne Rasband, NIH | RRID:SCR_003070 | Version 1.49 |