Figure 1.

Validation of the selectivity and extent of MOR knock-down in striatal subpopulations. Ai, Representative RNA in situ hybridization images for MOR (oprm1 in white), dopamine receptor 1 (D1 or drd1a in red) and dopamine receptor 2 (D2 or drd2 in green), are shown from the dorsolateral striatum of control, flMOR, and D1flMOR, D2flMOR, and A2aflMOR mouse lines. White arrows without a tail demonstrate D1-expressing cells and yellow arrows with a tail show D2-expressing cells. The cells marked by pink arrows in the A2aflMOR images show cells that are oprm1 and drd2 positive. Aii, Representative RNA in situ hybridization images of the dorsolateral striatum showing oprm1 (white), ChAT (green), and D1 (red) labeling in flMOR and ChATflMOR lines. Arrows highlight ChAT+ cells. Scale bar = 20 μm (Ai, Aii). B, Oprm1 expression were quantified and presented as the % colocalization for each genotype of MOR with D1+ cells in Bi, MOR with D2+ cells in Bii, and MOR with ChAT+ or D1+ cells in Bii; *p < 0.05, **p < 0.01, and ***p < 0.001 versus flMORs of the same experiment. C, qPCR for oprm1, drd1, and drd2 cDNA levels in striatal tissue shows reduced oprm1expression in D1flMORs (***p < 0.001) and D2flMORs (*p < 0.05) compared with flMORs. There was no effect of these MOR deletions on drd1 (Cii) or drd2 (Ciii) expression. Refer to Table 2 for statistical analyses. All data are shown as mean ± SEM, and the individual datapoints are shown in Extended Data Figure 1-1, for which this legend also applies.