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The primer sequences used in the qPCR validation of MOR knock-down
| Gene primer sequences (5’−3’) | |
|---|---|
| OPRM1 FWD | TCAAGGCCCTGGATTTCCGTACCC |
| OPRM1 RVS | CGGGCAGACCAATGGCAGAAGAGA |
| DRD1 FWD | CTTGTCTGTGCCGCTGTCATCAGG |
| DRD1 RVS | GGCATGACCAAGACAGCCACCAAG |
| DRD2 FWD | TTGTTCTTGGTGTGTTCATC |
| DRD2 RVS | TATAGATGATGGGGTTCACG |
| ACTB FWD | TGTGCACTTTTATTGGTCTC |
| ACTB RVS | GATGTATGAAGGCTTTGGTC |
OPRM1; μ-opioid receptor, DRD1; dopamine 1 receptor, DRD2; dopamine 2 receptor, ACTB; β-actin control, FWD; forward, RVS; reverse.
