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. 2020 Sep 18;7(5):ENEURO.0146-20.2020. doi: 10.1523/ENEURO.0146-20.2020

Table 5.

Statistical analyses of the IVSA profile in all lines (Fig. 4)

Item Figure Experiment Statistical test Effect or interaction Main effect flMOR D1flMOR D2flMOR A2aflMOR ChATflMOR
a 3A Remifentanil acquisition LMM AL day effect p < 0.0001, χ2 = 21.017 n = 15 n = 12 n = 10 n = 12 n = 12
b 3C Remifentanil acquisition LMM RNFS earned day effect p < 0.0001, χ2 = 19.132
c 3D Remifentanil acquisition LMM Percent AL presses day effect p < 0.01, χ2 = 9.730
d 3E Oxycodone maintenance LMM AL presses × Treatment Oxycodone vs. Saline p < 0.001, χ2 = 10.926 p < 0.001, χ2 = 11.601, n = 14 p < 0.01, χ2 = 6.764, n = 9 p < 0.001, χ2 = 12.806, n = 9 p < 0.01, χ2 = 10.545, n = 8 p < 0.001, χ2 = 13.68, n = 7
e 3G Oxycodone maintenance LMM RNFS × treatment Oxycodone vs. Saline p < 0.0001, χ2 = 16.051 p < 0.001, χ2 = 11.435 p < 0.01, χ2 = 8.434 p < 0.01, χ2 = 9.339 p < 0.001, χ2 = 10.981 p < 0.001, χ2 = 14.648
f 3G Oxycodone maintenance LMM Percent AL presses treatment × day oxycodone vs saline p < 0.0001, χ2 = 34.81 p < 0.0001, χ2 = 15.957 p < 0.0001, χ2 = 20.334 p < 0.01, χ2 = 7.918 p < 0.0001, χ2 = 16.952 p < 0.0001, χ2 = 19.941
g 3E Extinction; transition LMM AL presses treatment × day oxycodone vs. saline p < 0.01, χ2 = 8.296
h 3G Extinction; transition LMM RNFS treatment × day oxycodone vs. saline p < 0.01, χ2 = 7.047
i 3E Extinction; transition LMM AL presses
day effect within oxycodone treated
p < 0.0001, χ2 = 29.255
j 3F Extinction; transition LMM IAL presses
day effect within oxycodone treated
p < 0.01, χ2 = 9.396
k 3G Extinction; transition LMM RNFS day effect within oxycodone treated p < 0.0001, χ2 = 25.725
l 3G Extinction LMM AL presses × genotype within oxycodone treated p = 0.050, χ2 = 9.480 N.A. N.S. N.S. p = 0.043 N.S.
m 3G Extinction LMM IAL presses × genotype within oxycodone treated p = 0.057, χ2 = 9.148 N.A. N.S. N.S. p = 0.025 N.S.
n 3G Extinction LMM RNFS earned × genotype within oxycodone treated p = 0.018, χ2 = 11.934 N.A. N.S. N.S. p = 0.031 p = 0.007
o 3G Extinction LMM RNFS earned
day effect
p < 0.001, χ2 = 12.979 p < 0.001,
t(50) = –4.124
p < 0.001,
t(53) = –3.964
p < 0.001,
t(55) = –3.946
p < 0.001,
t(55) = –3.135
p < 0.001,
t(56) = –2.726
p 3I Last day AL oxycodone Two-way ANOVA Genotype × time F(480,5160) = 0.45, p > 0.99
q 3L Last day oxycodone RNFS Two-way ANOVA Genotype × time F(480,4920) = 0.2, p > 0.99
r 3J First day extinction AL Two-way ANOVA Genotype × time F(480,4920) = 1.44, p < 0.0001 Reference genotype N.S. N.S. p < 0.05 N.S.
s 3M First day extinction RNFS Two-way ANOVA Genotype × time F(480,4800) = 1.66, p < 0.0001 Reference genotype N.S. N.S. p < 0.05 p < 0.05
t 3J Third day extinction AL Two-way ANOVA Genotype × time F(480,5160) = 0.42, p > 0.99 Reference genotype N.S. N.S. N.S. N.S.
u 3M Third day extinction RNFS Two-way ANOVA Genotype × time F(480,5280) = 2.02, p < 0.0001 Reference genotype N.S. N.S. N.S. p < 0.05 and p < 0.01

N.S.: not significant, N.A: not applicable.