TABLE 1.

Impact of KPC-2 W105 mutations on reaction with vaborbactam

Enzyme mutation	k2/K (M−1 s−1)	koff (s−1)	Residence time (min)	Kd (nM)
WT	5.8 × 103 ± 0.3 × 103	4.3 × 10−5 ± 0.6 × 10−5	394 ± 50	7.4 ± 0.9
W105F	4.1 × 103 ± 0.1 × 103	3.9 × 10−5 ± 0.5 × 10−5	437 ± 59	9.3 ± 1.1
W105N	2.9 × 103 ± 0.1 × 103	5.0 × 10−5 ± 0.6 × 10−5	337 ± 46	17 ± 2
W105L	0.63 × 103 ± 0.01 × 103	2.3 × 10−5 ± 0.3 × 10−5	721 ± 101	37 ± 4
W105V	0.94 × 103 ± 0.1 × 103	3.5 × 10−5 ± 0.3 × 10−5	473 ± 41	38 ± 3
W105D	0.32 × 103 ± 0.07 × 103	3.5 × 10−5 ± 0.3 × 10−5	477 ± 48	110 ± 12
W105A	0.85 × 103 ± 0.07 × 103	7.7 × 10−5 ± 0.8 × 10−5	219 ± 23	90 ± 10
W105Y	3.8 × 103 ± 0.3 × 103	3.6 × 10−5 ± 0.2 × 10−5	463 ± 20	9.6 ± 0.8
W105S	0.92 × 103 ± 0.08 × 103	4.4 × 10−5 ± 0.6 × 10−5	386 ± 53	47 ± 6