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. 2020 Sep 21;64(10):e00398-20. doi: 10.1128/AAC.00398-20

TABLE 2.

Impact of KPC-2 W105 mutations on nitrocefin and meropenem hydrolysis

Enzyme mutation	Nitrocefin			Meropenem
Enzyme mutation	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ μM⁻¹)	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ μM⁻¹)
WT	43 ± 4	190 ± 6	4.40 ± 0.25	15 ± 2	3.8 ± 0.1	0.26 ± 0.02
W105F	71 ± 3	192 ± 13	2.70 ± 0.07	16 ± 2	1.8 ± 0.1	0.12 ± 0.01
W105N	160 ± 14	135 ± 17	0.84 ± 0.04	59 ± 5	3.1 ± 0.1	0.053 ± 0.003
W105L	357 ± 18	290 ± 4	0.81 ± 0.03	55 ± 4	2.5 ± 0.1	0.046 ± 0.002
W105V	236 ± 9	198 ± 8	0.84 ± 0.06	59 ± 7	4.2 ± 0.3	0.073 ± 0.007
W105D	558 ± 68	172 ± 10	0.31 ± 0.02	122 ± 4	15.7 ± 0.2	0.129 ± 0.003
W105A	299 ± 29	238 ± 3	0.80 ± 0.07	65 ± 10	2.8 ± 0.2	0.044 ± 0.004
W105Y	79 ± 5	247 ± 9	3.12 ± 0.11	19 ± 1	2.6 ± 0.04	0.14 ± 0.01
W105S	293 ± 11	169 ± 3	0.58 ± 0.02	64 ± 8	2.2 ± 0.2	0.035 ± 0.001