TABLE 5.

Kinetic parameters of KPC-2 inhibition by avibactam and vaborbactam^a

Enzyme mutation	Inhibitor	k2/K (M−1 s−1)	koff (s−1)	Residence time (min)	Kd (nM)
WT	Avibactam	2.3 × 104 ± 0.2 × 104	3.3 × 10−4 ± 0.1 × 10−4	51 ± 2	14 ± 1
S130G	Avibactam	8.6 ± 1.3	ND	ND	ND
WT	Vaborbactam	5.8 × 103 ± 0.3 × 103	4.3 × 10−5 ± 0.6 × 10−5	394 ± 50	7.4 ± 0.9
S130G	Vaborbactam	6.7 × 104 ± 0.3 × 104	4.5 × 10−3 ± 1.0 × 10−3	3.8 ± 1.0	67 ± 13

^aND, not determined.