TABLE 1.

Predicted peptides identified via mass spectrometry in enzymatic digests of LF and their putative physicochemical properties^a

Name	Position	Amino acid sequence	No. of amino acids	Mol wt	Net charge	GRAVYb	Boman indexc
PEP1	521–549	CALCAGDDQGLDKCVPNSKEKYYGYTGAF	29	3,117.47	−1	−0.48	1.32
PEP2	590–619	LLCLDGTRKPVTEAQSCHLAVAPNHAVVSR	30	3,186.7	+1	0.14	1.25
PEP3	216–251	KCLQDGAGDVAFVKETTVFENLPEKADRDQYELLCL	36	4,059.58	−4	−0.34	1.78
PEP4	263–299	ECHLAQVPSHAVVARSVDGKEDLIWKLLSKAQEKFGK	37	4,118.77	+1	−0.31	1.47
PEP5	252–289	NNSRAPVDAFKECHLAQVPSHAVVARSVDGKEDLIWKL	38	4,201.77	0	−0.28	1.79
PEP6	661–699	NDNTECLAKLGGRPTYEEYLGTEYVTAIANLKKCSTSPL	39	4,264.79	−1	−0.5	1.61
PEP7	550–588	RCLAEDVGDVAFVKNDTVWENTNGESTADWAKNLNREDF	39	4,430.74	−5	−0.81	2.79
PEP8	249–290	LCLNNSRAPVDAFKECHLAQVPSHAVVARSVDGKEDLIWKLL	42	4,644.39	0	0.07	1.24
PEP9	590–642	LLCLDGTRKPVTEAQSCHLAVAPNHAVVSRSDRAAHVKQVLLHQQALFGKNGK	53	5,714.61	+4	−0.18	1.52
PEP10	238–289	PEKADRDQYELLCLNNSRAPVDAFKECHLAQVPSHAVVARSVDGKEDLIWKL	52	5,876.66	−2	−0.46	2.06
PEP11	252–305	NNSRAPVDAFKECHLAQVPSHAVVARSVDGKEDLIWKLLSKAQEKFGKNKSRSF	54	6,038.88	+4	−0.61	2.23
PEP12	414–470	DGGYIYTAGKCGLVPVLAENRKSSKHSSLDCVLRPTEGYLAVAVVKKANEGLTWNSL	57	6,080.97	+2	−0.1	1.08
PEP13	653–708	SETKNLLFNDNTECLAKLGGRPTYEEYLGTEYVTAIANLKKCSTSPLLEACAFLTR	56	6,203.06	−1	−0.24	1.46
PEP14	291–347	SKAQEKFGKNKSRSFQLFGSPPGQRDLLFKDSALGFLRIPSKVDSALYLGSRYLTTL	57	6,366.33	+6	−0.43	1.81

^aMolecular weight, net charge, and GRAVY were determined by using the ProtParam tool. Boman index was determined by using ADP3. Potentially synergistic peptides PEP2 and PEP8 are shown in bold.

^bGrand average of hydropathy (GRAVY) is a prediction of the average hydrophobicity of a peptide. Positive numbers indicate the peptide is more hydrophobic, while negative numbers indicate the peptide is more hydrophilic.

^cThe Boman index is a prediction of the potential for a protein to bind to other proteins. Higher positive values indicate the ability to interact with a wide range of proteins.