FIG 5.

(A) Localization of the Aspergillus fumigatus Cy51A and Cyp51B proteins was assessed following 2 h of exposure to subinhibitory concentrations of the antifungal agents amphotericin B, caspofungin, voriconazole, and Congo red. White arrows indicate fluorescent apical regions of the hyphae, and yellow dotted arrows indicate fluorescent septa observed with a high frequency in Congo red-treated cultures. (B) Quantification of fluorescent apical tips and septa following treatment with the listed antifungals. Treatment with Congo red resulted in a notable increase in the fluorescence of the septa and the apical regions of the hyphae, while treatment with voriconazole and caspofungin resulted in an increased prevalence of fluorescent apical segments.