FIG 2.

Genetic engineering of AbaR curing in A. baumannii. (A) Schematic representation of AbaR11 inserted in the comM gene in A. baumannii AB5075 genome. All the sequences and genes in gray color belong to the wild-type AB5075 chromosome, the interrupted comM gene is, however, highlighted in dark blue. Transposons Tn6019 and Tn6018 constituting AbaR11 are indicated as in reference 4. For illustration purposes, the scale of the AbaR island is not respected. (B) Intermediary genetic construct with the counterselectable cassette replacing the AbaR11 island. (C) Final genetic structure of the locus after restoring the comM gene integrity.