TABLE 3.
Activity of posaconazole and VFV in virus yield reduction assays and in enzymatic assays in vitro
| Compound | Antiviral activity (μM) |
IC50 (μM)c | |
|---|---|---|---|
| EC50a | EC90b | ||
| PCZ | 2.2 ± 1.1 | 7.1 ± 1.1 | 8.0 ± 2.5 |
| VFV | 1.2 ± 0.9 | 10.1 ± 1.3 | 0.5 ± 0.2 |
| VCZ | NDd | ND | >100 |
Compound concentration that inhibits 50% of virus yield, as determined by titration in HFFs. Values are means ± SD of data derived from 4 independent experiments in duplicate.
Compound concentration that inhibits 90% of virus yield, as determined by titration in HFFs. Values are means ± SD of data derived from 4 independent experiments in duplicate.
Compound concentration that causes a 50% decrease in the rate of lanosterol conversion, as determined by reconstitution of the hCYP51 activity in vitro (2-min reaction). Values are means ± SD of data derived from 3 independent experiments in duplicate and calculated using GraphPad Prism 6.0 (dose response – inhibition).
ND, not determined.