Fig. 2.

Comparison between the Cas12a HITI, Cas12a MITI, and Cas9 HITI strategies-mediated reporter knock-in at the AAVS1 locus. a Schematic of the Cas9 HITI, Cas12a HITI and Cas12a MITI strategies at the AAVS1 locus. The dotted lines represent the cleavage site of Cas9 or Cas12a. The sequence of the cleavage site is shown in vermilion for Cas12a MITI and in orange for Cas12a HITI. The protospacer adjacent motif (PAM) sequence of Cas12a is shown in green, and the PAM sequences of Cas9 are in blue. b The precise integration efficiencies of the three knock-in strategies at the AAVS1 locus. The result was presented as mean ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t test