Fig. 3.

Tagging the CLTA gene using the Cas12a MITI or Cas9 HITI strategy in HEK293T cells. a Schematic of CLTA gene targeting using the two approaches. Red “TGA” base pairs are the termination codon of the CLTA gene. b Relative precise knock-in efficiency of the two strategies in HEK293T cells at the CLTA locus tested via the percentage of tdTomato positive cells evaluated by flow cytometry analysis. c The precise integration efficiencies of the two knock-in strategies at the CLTA locus via TA cloning and Sanger sequencing. All the results were presented as mean ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, unpaired Student’s t test