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. 2020 Sep 22;11:4775. doi: 10.1038/s41467-020-18594-3

Fig. 3. DMA-135 inhibits EV71 IRES-dependent translation and replication.

Fig. 3

a The diagram depicts the bicistronic reporter plasmid used to synthesize RNA for transfections and dual-luciferase assays. b Effect of DMA-135 on EV71 IRES activity. SF268 cells were transfected with RLuc-EV71-5′UTR-FLuc RNA and cultured with various concentrations of DMA-135. Luciferase activity was measured 2 days later. Mean values ± standard deviations from three independent experiments (N = 3) are shown in the bar graphs. P values were determined by unpaired two-tailed Student’s t test. ***P < 0.001; **P < 0.01; N.S., not significant. 0.001 µm: P = 0.867669; 0.1 µm: P = 0.006658; 0.25 µm: P = 0.000011; 0.5 µm: P = 0.000305; 1 µm: P = 0.000031; 10 µm: P < 0.00001; 50 µm: P < 0.00001; 100 µm: P < 0.00001; 250 µm: P < 0.00001; 500 µm: P < 0.00001; 1000 µm: P < 0.00001. c Effect of DMA-135 on EV71 replication. SF268 cells were infected with EV71 at an moi = 1. Various concentrations of DMA-135 were added to the cells. Media were harvested 24 hr post infection and assayed for infectious virus by plaque formation in Vero cells. Mean values ± standard deviations from three independent experiments (N = 3) are shown. P values were determined by unpaired two-tailed Student’s t test. 0.001 µm: P = 0.025719; 0.1 µm: P = 0.153792 (N.S.); 0.25 µm: P = 0.000485; 0.5 µm: P = 0.000171; 1 µm: P = 0.000167; 10 µm: P = 0.000167; 50 µm: P = 0.000167.