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. 2020 Sep 22;11:4775. doi: 10.1038/s41467-020-18594-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Calorimetric titrations of AUF1-RRM1,2 to SLII or to SLII:DMA-135 complex at different molar ratios reveal a single transition in the binding isotherm characterized by an enthalpically driven molecular recognition event. All data were processed and analyzed using Affinimeter²⁵. The processed thermograms were fitted to a 1:1 stoichiometric binding model, represented by the red lines. Values for dissociation constants (K_D) and corresponding standard deviation are reported for each titration. The experiments were performed in 10 mm K₂HPO₄, 20 mm KCl, 0.5 mm EDTA, and 4 mm BME (pH 6.5) buffer at 298 K. b Graphical representation of the dissociation constants for the AUF1-RRM1,2-SLII complex as a function of DMA-135 concentration. Reported K_D values and corresponding standard deviations are from n = 3 experimental replicates. Data represented as mean ± SD of triplicate experiments. Uncertainties in individual binding isotherms are calculated using Affinimeter and are determined by considering the noise and quality of raw data and the dispersion of the integrated signal as a function of concentration.