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. 2020 Sep 22;10:15443. doi: 10.1038/s41598-020-72076-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of EECGL on hepatic and cardiac marker enzymes, lipid contents, serum inflammatory factors and tissue architecture. (A) hepatic marker enzymes, (B) LDH, (C) CK-MB, (D) cardiac troponin I and T, (E) hepatic lipid content, (F) cardiac lipid content, (G) hepatic FFA, (H) cardiac FFA, (I) IL-1β, (J) TNF-α and TGF-β, (K) IL-6 and IL-10. The liver and heart pathologies in the different groups of HLD, MSG, HM and HM + EECGL fed rats were observed. Bright field microscopy images of liver, heart tissue by (L,M) Hematoxylin and eosin (HE) staining (20 ×) and (N,O) Oil Red O staining (20 ×). Significance level based on Mann–Whitney U multiple comparison test: a-NC vs. HLD, b-NC vs. MSG, c-NC vs. HM, d-HLD vs. MSG, e-HLD vs. HM, f-MSG vs. HM, g-HM vs. HM + EECGLL, h-HM vs. HM + EECGLM, i-HM vs. HM + EECGLH, j-HM + EECGLL vs. HM + EECGLM, k-HM + EECGLL vs. HM + EECGLH, l-HM + EECGLM vs. HM + EECGLH [*P < 0.05, **P < 0.01, ***P < 0.001].