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. 2020 Sep 22;10:15443. doi: 10.1038/s41598-020-72076-6

Figure 7.

Figure 7

Protein expression in hepatocytes and cardiomyocytes in control, HM and HM + EECGLH group. Effects of EECGLH on HM induced alerted protein expressions of (A,B) hepatocytes and (C,D) cardiomyocytes with control groups with densitometric analysis some cell cycle regulator protein like p53 and p21, some transcription factors like PPAR-α and PPAR-γ, apoptosis related protein Bcl2, Bax, cleaved caspase 3 and 9 with an endogenous control GAPDH were represented. Gene expression in hepatocytes and cardiomyocytes in control, HM and HM + EECGLH group. Effects of EECGLH on HM induced alerted mRNA expression levels of (E) hepatocytes, (F) cardiomyocytes with control groups were represented and (G) bar diagram represented the densitometric analysis of TNF-α, iNOS, and eNOS with β-actin as endogenous control. Significance level based on Mann–Whitney U multiple comparison test: c-NC vs. HM, i-HM vs. HM + EECGLH [**P < 0.01].