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. 2020 Sep 22;11:4792. doi: 10.1038/s41467-020-18554-x

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© The Author(s) 2020, corrected publication 2022

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Fig. 3 — a The apparent binding constant (k_b) of ATP and Cu²⁺ ions derived from the plot of [ATP]/Δε_{a,259 nm} vs. [ATP] at various concentrations (5–100 μM) for Cu(OTf)₂ (5 μM). b EPR spectra of ATP, Cu²⁺ and Cu²⁺·ATP. Experimental conditions: ATP (50 mM), Cu(OTf)₂ (10 mM), glycerol (20 v/v%), MES (20 mM, pH 5.5), 100 K. c ¹H NMR and d ³¹P NMR spectroscopic titrations of ATP (120 mM) by varying the concentration of CuCl₂ (0.12–24 mM) in D₂O.