Table 2.

Kinetic study of Cu²⁺·ATP.

Entrya	Catalyst	kapp (M−1 s−1)	krel
1	None	(1.6 ± 0.1) × 10−3	1.0
2	ATP	(1.3 ± 0.3) × 10−3	0.8
3	Cu(OTf)2	(1.1 ± 0.1) × 10−2	6.9
4	Cu2+·ATP	(2.0 ± 0.1) × 10−2	12.5

^aReaction conditions: 1a (20–50 μM), 2 (5 mM), ATP (250 μM), Cu(OTf)₂ (50 μM), MES buffer (2 mL, 20 mM, pH 5.5), 4 °C. All kinetic measurements were performed in triplicate and standard deviations were calculated. The rate acceleration k_rel was calculated as the ratio of k_app,cat/k_app,uncat, where k_app,uncat and k_app,cat are the k_app values in the absence and presence of the catalyst, respectively.