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. 2020 Sep 22;11:4788. doi: 10.1038/s41467-020-18524-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Immunoblots showing the expression of the indicated Vav2 proteins in human keratinocytes (top panel). Tubulin was used as loading control in all cases (bottom panel) (n = 3 independent experiments). p1 and p2 refer to two independent pools of cells. b Tyrosine-phosphorylation levels (top panel) of immunoprecipitated Vav2^WT (bottom panel) in the keratinocyte cell lines. Similar data were obtained in two additional independent experiments. p, tyrosine-phosphorylated. IgG, immunoglobulin band derived from the antibody used in the immunoprecipitation step. The asterisk marks a high molecular weight tyrosine-phosphorylated protein that coimmunoprecipitates with Vav2. c Representative images of hematoxylin/eosin-stained organotypic cultures generated by the indicated cells (top). The thickness of the corneum and cellular epidermal strata is indicated with red and blue brackets, respectively. Scale bar, 10 μm (n = 3 independent cultures). d Thickness of the cellular and corneum strata using data from c. *P = 0.013; ***P < 0.0001 (ANOVA and Dunnett’s multiple comparison test, n = 3 independent cultures). Data represent the mean ± SEM. Source data for this figure are provided as a Source Data file.