. 2020 Sep 22;11:4774. doi: 10.1038/s41467-020-18574-7

Table 1.

Key properties of representative polymerase-free and PCR-based technologies.

	Polymerase-free				PCR-based
	SMOLT	Simoa^13,27,36	nCounter¹⁷	Electrochemical sensor¹⁴	Xpert^37–39	T2Dx^9,10
LOD for single-stranded nucleic acid (fM)	0.1 (whole blood)	0.1 (buffer)	0.1–0.5 (cell culture)	–	7·10⁻⁷ (plasma)	–
LOD for microbe in whole blood (CFU mL⁻¹)	1–3	4·10⁴	–	10⁷	10	1–11
Turnaround time (h)	1.5	5.5	>20	1	1.5–2	4–8
Multiplexing (number of targets)	>9	10	>500	16	3	5
Instrumentation cost	Low	High	High	Low	High	High