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. 2020 Sep 22;10:15412. doi: 10.1038/s41598-020-71907-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Messenger RNA levels and photosynthetic behavior of mutants for components of (de)methylation pathways. (a) Heatmap showing relative changes in the mRNA expression of genes involved in the RdDM pathway and of genes selected because of their mRNA expression profile in an Arabidopsis cell culture after the onset of light in a time series of 1 to 14 days²⁸. Log₂-fold changes are calculated relative to gene expression values at day 0. (b) The maximum quantum yield of PSII (F_v/F_m) was measured with an imaging Chl fluorometer (Imaging PAM). Seedlings were grown on half-strength MS medium for 4 days under LD conditions, and were dark-adapted for 10 min before transient chlorophyll fluorescence was measured. The sig6-1 and gun4-2 mutants were used as controls. Data represent mean values from at least 20 seedlings. Significant differences (t-test; p < 0.05) with respect to the WT are indicated by asterisks.