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. 2020 Sep 22;11(9):785. doi: 10.1038/s41419-020-02997-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a FAS expression in A549 and H292 lung cancer cells after overexpressing miR-196b-5p was determined by qRT-PCR. Results are represented as means ± SD (n = 3). b Western blot analysis of FAS protein in A549 and H292 lung cancer cells after overexpressing miR-196b-5p. The bands were quantified using Image J software, and relative values were obtained by normalizing to the value of each corresponding GAPDH. c Expression level of FAS in 60 paired NSCLC tissues and their matched NATs. The RNA samples were extracted from 30 NSCLC tissues and 30 corresponding NATs. The RNAs were subject to qRT-PCR with a FAS probe, and the expression was normalized by GAPDH. d Upper panel shows representative immunohistochemical staining for FAS in NSCLC tissues and normal adjacent tissues (NATs) from the same patient. Summary of tissue immunohistochemical staining data for FAS in 48 pairs of clinical NSCLC tissues and NATs are shown in the lower panel. **P < 0.01, ***P < 0.001.