Fig. 7. ST2 controls macrophage cell cycle progression and activation in vitro.

a–c Bone marrow-derived cells were cultured in M-CSF alone (unstimulated) or cultured in the presence of M-CSF with varying concentrations of IL-13 and IL-33 for 3 days ex vivo. a Representative histograms from WT and ST2^−/− BMDMs showing DAPI staining after fixation. Cell cycle phases (G0/G1, S, and G2) are gated by nuclear DNA content. b Quantification of BMDMs in the three cell cycle phases and shown in (a) and disc graphs denote mean only. c Heat-map constructed from Fluidigm analysis of mRNA transcripts for the denoted genes from cultured WT and ST2^−/− BMDMs. Scale bar on the bottom denotes relative log₂ differences in gene expression for each row. Samples are bone marrow treated and cultured separately from three individual mice per genotype. All bar graphs show means ± SEM. Data from n = 3 mice are representative from two independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001 between indicated IL-33-treated and unstimulated BMDMs. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001 between IL-33-stimulated ST2^−/− and WT BMDMs using two-way ANOVA, Bonferroni post-test.