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. 2020 Sep 22;11:4786. doi: 10.1038/s41467-020-18466-w

Fig. 8. IL-33 synergizes with IL-13 to ensure a matured macrophage repairing phenotype.

Fig. 8

a–g Bone marrow-derived cells from WT Balb/c, ST2−/− (Balb/c background), or ST2-GFP (Balb/c) mice were cultured in M-CSF alone (unstimulated), or cultured in the presence of M-CSF with varying concentrations of IL-13 and/or IL-33 for 6 days ex vivo. a Representative flow cytometric plots of CD206 and GFP expression from ST2 GFP bone marrow-derived macrophages (BMDMs). Numbers next to gate denote percentage of CD206+ ST2 GFP+ BMDMs. b Quantification of the frequency of CD206+ GFP ST2+ gated in (a). c Representative flow plots of Arginase-1 (Arg-1) and CD206 expression on WT and ST2−/− BMDMs. Gates and numbers denote the percentage of CD206+ Arg-1+ BMDMs. d Quantification of the frequency of CD206+ Arg-1+ BMDMs from WT or ST2−/− mice as gated in (c). e–g Quantification of HGF (e), IGF-I (f), and BRP-39 (g) from the supernatants of WT and ST2−/− BMDMs. Samples are bone marrow treated and cultured separately from three individual mice per genotype. Bar graphs from n = 4 (b), 3 (d–g) mice, show mean ± SEM pooled from three independent experiments.