Liposomal-echinomycin Reduces Metastasis of MDA-MB-231 and SUM-159 in Xenograft Mice. A-F) NSG mice were transplanted with 1.0x106 MDA-MB-231 (A-D) or SUM-159 (E-F) and treated with CrEL-echinomycin, liposomal-echinomycin or vehicle and euthanized as described in Figure 3A&B. Fixed lung and liver tissues were stained with H&E or anti-human Vimentin antibody. Representative H&E staining (B, D, F, top panels) and immunofluorescence staining for human Vimentin (B, D, F, bottom panels) are shown for fixed lung (B&F) and liver (D) tissues for mice xenografted with MDA-MB-231 (B&D) or SUM-159 (F) tumors. Summarized data corresponding to B, D and F are shown in A, C, and E, respectively, displaying mean number of metastatic nodules ± SEM found in lung and liver tissues.