Fig. 3.

a ¹⁵N HSQC spectra of PioC obtained with an R₂-weighted ¹⁵N-HSQC-AP experiment collected with T = 2.4 ms (blue) and the first point of the R₂ series collected with the standard experiment with an overall relaxation delay = 12.5 ms (red). Experiments were recorded using a 500 MHz NEO-Avance Bruker spectrometer equipped with Triple resonances inverse cryoprobe (CP-TXI). R₂-weighted ¹⁵N-HSQC-AP was recorded with 256 scans each fid using an overall recycle delay of 200 ms, the in-phase experiment with 16 scans each fid and 4 s as recycle delay. Folded peaks are marked with an asterisk. b Expanded plot of selected rows of R₂-weighted ¹⁵ N-HSQC-AP, corresponding to His7 and Asn27