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. 2020 Sep 22;10:15418. doi: 10.1038/s41598-020-72165-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of drugs on the autolytic process. (A) Percent changes in O.D. were measured in cultures of S. pneumoniae exposed to various concentrations of drugs expressed as multiples of MIC. Percent changes were calculated by comparing initial and final O.D. values. (B) O.D. changes in cells treated with vancomycin (VAN) in the presence and absence of HC-3 (HC3), MN58b (MN), and RSM-932A (RSM). (C) Detergent induced lysis of cells grown to saturation in the presence of 0.5 MIC MN58b and RSM-932A. (D) Phosphate induced lysis of cells grown to saturation in the presence of 0.5 MIC MN58b and RSM-932A.