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. 2020 Sep 22;10:15418. doi: 10.1038/s41598-020-72165-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the drugs on LTA production. (A) Western blot of total LTA from S. pneumoniae cells grown in the presence or absence of MN58b (MN) or RSM-932A (RSM). The western blot is cropped to focus on LTA species. Different species of LTA are numbered 1–5, with 1 being the largest in molecular weight and 5 being the smallest (see Supplementary Material for full western). (B) Total protein signal from the same membrane, used as loading and membrane transfer control. (C) Analysis of fold changes in the different species of LTA (1–5). 1 × fold change is marked with a dotted line. (D) Total fold change of LTA. 1 × fold change is indicated with a dotted line.