Table 2.
Dissociation constants (Ki), alpha coefficient values and ratio of Michaelis–Menten kinetic constants Km and Vmax constants derived from substrate-inhibitor competition assays. (A) Inhibitor vs ATP (B) Inhibitor vs choline.
| Ki (μM) | α | Kminh/Kmfree | Vmaxinh/Vmaxfree | Mechanism of Inhibition | |
|---|---|---|---|---|---|
| (A) | |||||
| RSM-932A | 35.8 ± 3.2 | 1.1 ± 0.17 | 0.92 | 0.61 | Noncompetitive |
| MN58b | 182.3 ± 28.1 | 6.0 ± 2.2 | 3.48 | 0.76 | Mixed |
| (B) | |||||
| RSM-932A | 10.6 ± 0.9 | 8.1 ± 1.8 | 3.08 | 0.76 | Mixed |
| MN58b | 123.8 ± 19.2 | a = 59.2 ± 90.9 | 2.3 | 0.69 | Mixed |