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. 2020 Sep 22;11(9):786. doi: 10.1038/s41419-020-03000-z

Fig. 2. Bip inhibition promoted IR-mediated ER stress and ICD generation.

Fig. 2

a Bip expression was detected using western blot in GSCs and non-GSCs after cells were irradiated with 10 Gy. b EGCG or siRNA-Bip enhanced the increase of IR-induced ER stress associated protein in GSCs. GSCs were transfected with siRNA-Bip or pcDNA-Bip. After pretreatment with or without 2.5 mM TUDCA for 6 h, cells were irradiated with 10 Gy, then indicated protein expressions were detected by western blot. c The level of CRT exposure on cell surface was measured using flow cytometry, and MFI was analyzed. Cells were transfected or untransfected with siRNA-Bip following 10 Gy radiation. d The level of extracellular ATP was measured using a chemiluminescence assay. e HMGB1 in supernatant was detected using an elisa assay. *P < 0.05, **P < 0.01 vs control, #P < 0.05, ##P < 0.01 vs IR.