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. 2020 Sep 22;3:523. doi: 10.1038/s42003-020-01253-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a TP114 annotated map (top panel) and transposon insertions sites (lower panel) obtained from different high-density transposon mutagenesis (HDTM) mutant libraries. Gene essentiality is highlighted in yellow boxes for plasmid maintenance, blue boxes for in vitro transfer and red boxes for in situ transfer and summarized in the “Essentiality” track (track 1 to 3 n = 3, biologically independent samples, track 4 to 7 n = 6, biologically independent mice). b The role of the type IV pilus major pilin subunit gene (pilS) in MPS was confirmed in vitro by conjugation in stable (solid) and unstable (liquid) conditions (n = 3, biologically independent samples). Error bars show standard deviation of the mean from at least 3 biological replicates, which are shown on graph as white circles. c TP114ΔpilS mutant in situ transfer was followed for 4 days in feces then (d) compared with efficiencies from the caecum (n = 5, biologically independent mice).