Fig. 4. TP114 transfer is activated under hypoxic conditions.

a, ratio between the transfer rate of the 13 conjugative plasmids in situ after 24 h (n = 4, biologically independent mice) and their transfer efficiency on agar (n = 3, biologically independent samples) (dark gray) or in broth (light gray) (n = 3). Since R388, pRts1, RK24, pIP69, TP123, and pVCR94 did not produce any transconjugant in situ, their transfer rate fold change was calculated using the experimental limit of detection. Individual data points are shown as white circles on graph. b, influence of the temperature, presence of feces and hypoxic environment on the transfer rates of TP114 in relation to transfer rates for 2 h at 37 °C on agar (n = 3, biologically independent samples). a and b, error bars represent the standard deviation of each dataset.