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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Exp Eye Res. 2020 Jul 25;198:108121. doi: 10.1016/j.exer.2020.108121

Fig. 1.

Fig. 1

Serpinf1 and Pnpla2 expression in retinal nuclear layers. The RPE, ONL, INL and GCL were isolated from WT and R91W/Nrl−/− using laser capture microdissection. Relative gene expression was determined by semiquantitative real-time PCR in each individual layer. Photos of agarose gels stained with Et-Br are shown and samples of retinal nuclear layers, the gene analyzed, and mouse line are indicated at the top. DNA size markers are M. Expression of Slc16a8 (marker for RPE), Opn1mw (marker for ONL), Vsx2 (marker for INL), and Opn4 (marker for GCL) was determined to monitor contamination between the isolated retinal layers. Actb served as housekeeping gene control. GCL= Ganglion cell layer, INL= Inner nuclear layer, ONL= Outer nuclear layer, RPE= Retinal pigment epithelium.