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. 2020 Sep 22;10:15424. doi: 10.1038/s41598-020-72022-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of maternal exercise during pregnancy on offspring liver energy sensing and on AMPK signaling in early life. (A) pAMPK/AMPK protein expression. (B) PPARα protein expression. (C) pACC/ACC protein expression. (D) Pgc1α mRNA expression. (E) Cpt1a mRNA expression. (F) Acc1 mRNA expression. (G) Acc2 mRNA expression. Representative immunoblots are presented above the respective graph. Immunoblots: CO (n = 5), INT (n = 5). Uncropped images of original blots are shown in Supplementary Fig. 6. mRNA Expression: CO (n = 5), INT (n = 7). Mean ± SEM; *p < 0.05, **p < 0.01. CO control, INT intervention, P postnatal day, AMPK adenosine monophosphate-activated protein kinase, PPARα peroxisome proliferator-activated receptor alpha, ACC acetyl-CoA carboxylase, GAPDH Glycerinaldehyd-3-phosphate-dehydrogenase, Pgc1α peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Cpt1a carnitine palmitoyltransferase 1A.