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. 2020 Sep 8;23(9):101544. doi: 10.1016/j.isci.2020.101544

Figure 6.

Figure 6

Localization of ILC2s and IL-33+ Cells in the Liver after Ameba Injection

(A) The liver sections from uninfected WT and Rag2 KO mice were stained with anti-T1/ST2 (red), anti-Gata3 (blue), and anti-CD31 (green) mAbs.

(B) Immunofluorescent staining with isotype control Ab and DAPI (white).

(C) The liver section from uninfected WT mice was stained for ILC2s and IL-33+ cells with anti-T1/ST2 (red), anti-Gata3 (blue), and anti-IL-33 (green) mAbs.

(D) The liver section from infected WT mice was stained for ILC2s and IL-33+ cells using anti-T1/ST2 (red), Gata3 (blue), and IL-33 (green) mAbs. Scale bar, 50 μm. Yellow dotted lines show the necrotic lesion of ALA. Numbers in white dotted squares show further detail on the right images as detail 1 and 2. Results are representative of three sections from three mice examined.