. 2020 Sep 9;11:2090. doi: 10.3389/fmicb.2020.02090

TABLE 2.

Summary of diversity index for microbial communities based on 16S rRNA and ITS1 gene sequencing data (cutoff = 0.005).

Gene	Sample group	Sample ID	Clean reads	Alpha diversity metrics
				OTU number	Chao1	Shannon
16S rRNA gene	Fermented grains from 6-years cellars	S0	85,411.33 ± 24,774.82^a	132.667 ± 5.508^e	161.928 ± 9.316^de	2.955 ± 0.095^c
		S7	88,674.00 ± 25,556.67^a	668.000 ± 9.539^a	703.017 ± 25.707^a	4.645 ± 0.144^a
		S15	86,759.00 ± 11,543.91^a	650.667 ± 17.786^a	683.005 ± 16.981^a	4.209 ± 0.571^a
		S25	77,252.67 ± 7,914.13^a	584.333 ± 75.659^ab	654.738 ± 62.682^a	3.069 ± 1.230^b,c
		S45	72,487.67 ± 17,313.82^a	479.667 ± 19.731^c,d	586.012 ± 43.211^a,b	1.631 ± 0.527^d,e
		S70	79,761.00 ± 20,257.24^a	411.333 ± 76.159^d	529.301 ± 69.223^b,c	0.964 ± 0.355^e
		S95	61,048.33 ± 21,817.11^a	173.667 ± 11.060^e	221.071 ± 38.751^d	0.928 ± 0.341^e
	Fermented grains from 30-years cellars	T0	77,209.00 ± 20,652.86^a	144.000 ± 6.083^e	179.667 ± 16.966^d,e	2.753 ± 0.114^c
		T7	73,004.67 ± 14,485.12^a	523.667 ± 116.663^b,c	640.004 ± 14.256^a,b	4.057 ± 0.371^a,b
		T15	85,475.00 ± 11,085.05^a	595.000 ± 17.088^a,b	640.912 ± 19.105^a,b	4.117 ± 0.535^a,b
		T25	75,747.00 ± 20,257.17^a	589.000 ± 60.108^{a –c}	669.686 ± 34.141^a	4.143 ± 0.858^a,b
		T45	84,497.00 ± 21,211.93^a	523.000 ± 42.462^b,c	600.857 ± 48.286^a	2.477 ± 0.568^c,d
		T70	85,070.33 ± 33,460.74^a	381.667 ± 142.759^d	449.071 ± 187.814^c	1.328 ± 0.262^e
		T95	50,453.67 ± 24,837.44^a	88.000 ± 15.716^e	107.079 ± 4.423^e	0.793 ± 1.025^e
ITS1 gene	Fermented grains from 6-years cellars	S0	127,777.30 ± 29,252.08^a	62.000 ± 5.292^f	69.109 ± 6.891^d	1.562 ± 0.118^{c –f}
		S7	96,689.00 ± 21,542.83^a,b	95.000 ± 7.211^{a –d}	106.042 ± 12.237^{a –c}	1.577 ± 0.143^{c –f}
		S15	99,241.67 ± 14,200.82^a,b	116.000 ± 12.124^a	128.607 ± 5.380^a	2.129 ± 0.758^b,c
		S25	93,188.33 ± 4,787.20^a,b	101.000 ± 9.000^{a –c}	113.810 ± 17.829^a,b	1.834 ± 0.217b^{c –f}
		S45	110,710.00 ± 11,894.14^a,b	98.333 ± 15.373^{a –c}	101.044 ± 15.399^{a –c}	2.117 ± 0.083^b,c
		S70	96,658.67 ± 22,447.64^a,b	106.667 ± 4.509^{b –e}	108.709 ± 4.652^{a –c}	2.356 ± 0.147^b
		S95	84,384.67 ± 34,498.28^b	96.667 ± 16.196^{a –c}	102.700 ± 20.429^{a –c}	2.858 ± 0.339^a
	Fermented grains from 30-years cellars	T0	132,562.00 ± 12,909.96^a	68.000 ± 5.196^e,f	82.423 ± 4.759^c,d	1.521 ± 0.078^{d –f}
		T7	79,332.33 ± 11,985.76^b	109.333 ± 1.528^a,b	115.365 ± 6.281^a,b	2.045 ± 0.153b^c,d
		T15	134,318.30 ± 12,019.16^a	88.667 ± 18.452^{b –e}	97.225 ± 27.627^{b –d}	1.872 ± 0.137^{b –e}
		T25	106,741.00 ± 26,865.53^a,b	87.667 ± 18.771^{b –e}	103.988 ± 24.452^{a –c}	1.262 ± 0.220^f
		T45	109,846.30 ± 23,501.40^a,b	84.000 ± 5.568^{c –e}	93.302 ± 6.918b^c,d	1.308 ± 0.156^e,f
		T70	98,865.67 ± 29,523.02^a,b	88.667 ± 8.505^{b –d}	100.917 ± 16.962^{a –c}	1.701 ± 0.563^{c –f}
		T95	84,582.67 ± 26,941.50^b	73.333 ± 17.616^{d –f}	81.423 ± 20.529^c,d	1.489 ± 0.133^{d –f}

All data are presented as means ± standard deviations (n = 3). Values with different letters in a column mean significant differences at p < 0.05 as determined by one-way ANOVA Duncan’s test.