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. 2020 Sep 9;37:101716. doi: 10.1016/j.redox.2020.101716

Fig. 4.

Fig. 4

Overexpression of human recombinant SOD2 (rhSOD2) restores ROS levels and mitochondrial function in DGCR8 knockdown hMSCs. (A) The mRNA levels of SOD genes were analysed by quantitative real-time PCR. Data were normalised to GAPDH mRNA levels. Error bars indicate the standard error of the mean of three independent experiments (*P < 0.05). (B) Immunoblot analysis of SOD proteins in DGCR8 knockdown cells. (C) Total ROS levels were measured in siDGCR8-transfected cells after rhSOD2 overexpression (5 μg/mL), using H2DCF-DA. Error bars indicate the standard error of the mean of three independent experiments (**P < 0.01). (D) Western blot showing the protein levels of Ras signalling components in rhSOD2-overexpressing DGCR8 knockdown hMSCs.